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WANG Guixing, ZHANG Xiaoyan, SUN Zhaohui, ZHAO Yaxian, DU Wei, CUI Jianbin, HOU Jilun, WANG Yufen. Induction of gyno-tetraploidy in Japanese flounder Paralichthys olivaceus[J]. Journal of Oceanology and Limnology, 2020, 38(1): 288-293

Induction of gyno-tetraploidy in Japanese flounder Paralichthys olivaceus

WANG Guixing1,2, ZHANG Xiaoyan2, SUN Zhaohui2, ZHAO Yaxian2, DU Wei2, CUI Jianbin3, HOU Jilun2, WANG Yufen2
1 Key Laboratory of Aquatic Genomics, Ministry of Agriculture, Beijing 100141, China;
2 Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China;
3 Ocean College, Agricultural University of Hebei, Qinhuangdao 066009, China
Tetraploid fish are important for mass production of triploids in polyploid breeding. In this study, we reported a novel protocol for artificial induction of tetraploidy by a combination of cold shock and hydrostatic pressure administered to gynogenetically developed eggs in Japanese flounder Paralichthys olivaceus. The induction was carried out by activating the eggs with UV-irradiated sperm of red sea bream Pagrus major, administering a cold shock (0℃, 5 to 45 min) 3 min after fertilization to inhibit second polar body exclusion, incubating the eggs for 60 min at 17℃, and treating them with a 650 kg/cm2 hydrostatics pressure shock for 6 min. We named the embryos gyno-tetraploids that developed from eggs after such treatments. The hatching rate of the gyno-tetraploids ranged from 20.99%±3.66% to 36.01%±2.79%, and the tetraploid rate ranged from 80.00% to 100.00%. All-maternal inheritance was verified using 6 high-recombination-rate microsatellite markers. This method successfully induced gyno-tetraploidy. The successful induction of gyno-tetraploidy lays the foundation for triploidization of new varieties with improved economic traits of interest that can benefit commercial culture.
Key words:    gynogenesis|polyploidy|chromosome manipulation|mosaic   
Received: 2018-09-24   Revised:
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