Cite this paper:
ZHANG Lei, CAI Yanan, JIANG Miao, DAI Jing, GUO Xiyao, LI Wei, LI Yuehong. The levels of microbial diversity in different water layers of saline Chagan Lake, China[J]. HaiyangYuHuZhao, 2020, 38(2): 395-407

The levels of microbial diversity in different water layers of saline Chagan Lake, China

ZHANG Lei1, CAI Yanan1, JIANG Miao2, DAI Jing1, GUO Xiyao1, LI Wei3, LI Yuehong1
1 College of Animal Science and Technology, Jilin Agriculture University, Changchun 130118, China;
2 Biomarker Technologies Co. Ltd., Beijing 100000, China;
3 Department of Clinical Laboratory, China-Japan Union Hospital, Changchun 130000, China
Abstract:
Saline lakes represent a particularly interesting aquatic environment for harboring high microbial diversity. However, the microbial diversity in different states and locations of saline lake is often overlooked. We studied for the first time the diversity and relative composition of the microbial communities in the Chagan Lake, NE China, and investigated the differences in microbial species and physical and chemical factors in different geographical localities of the lake. After extracting the total DNA of the sample, we tested the library with the established library, sequenced the qualified library with Illumina HiSeq 2500, and studied the bacterial diversity by 16S rRNA targeted metagenomics analysis. Results reveal that the highest microbial abundance in Chagan Lake at genus level was Proteobacterium followed by Actinomycetes and Bacteroides. In addition, we compared the microbial composition within the lake using alpha- and beta-diversity indices, showing that both diversity and evenness were the highest in the middle of the lake and lowest in the west of lake areas, and in the upper, middle, and lower depth of water columns, the low water column had the highest species number in the whole water environment, but the difference was not significant. We believe that physicochemical factors contribute to the formation of microbial community composition and diversity. In aquaculture industry, it is impossible apply horticulture for making full use of the spatial differences in the microbial composition of the water. Therefore, combining cultured aquatic animal with the most suitable microbial species is a good way to boost the breeding effect for greater economic value.
Key words:    saline lake|aquatic environment|microbial diversity|physical and chemical factors|Chagan Lake   
Received: 2019-01-30   Revised: 2019-04-08
Tools
PDF (2267 KB) Free
Print this page
Add to favorites
Email this article to others
Authors
Articles by ZHANG Lei
Articles by CAI Yanan
Articles by JIANG Miao
Articles by DAI Jing
Articles by GUO Xiyao
Articles by LI Wei
Articles by LI Yuehong
References:
Abed R M M, Ramette A, Hübner V, De Deckker P, De Beer D. 2012. Microbial diversity of eolian dust sources from saline lake sediments and biological soil crusts in arid Southern Australia. FEMS Microbiology Ecology, 80(2):294-304.
Abia A L K, Ubomba-Jaswa E, Momba M N B. 2015. Impact of seasonal variation on Escherichia coli concentrations in the riverbed sediments in the Apies River, South Africa.Science of the Total Environment, 537:462-469.
Al-Thukair A A, Abed R M M, Mohamed L. 2007. Microbial community of cyanobacteria mats in the intertidal zone of oil-polluted coast of Saudi Arabia. Marine Pollution Bulletin, 54(2):173-179.
Berry D, Xi C W, Raskin L. 2006. Microbial ecology of drinking water distribution systems. Current Opinion in Biotechnology, 17(3):297-302.
Besemer K, Singer G, Quince C, Bertuzzo E, Sloan W, Battin T J. 2013. Headwaters are critical reservoirs of microbial diversity for fluvial networks. Proceedings of the Royal Society B:Biological Sciences, 280(1771):20131760.
Casamayor E O, Schäfer H, Bañeras L, Pedrós-Alió C, Muyzer G. 2000. Identification of and spatio-temporal differences between microbial assemblages from two neighboring sulfurous lakes:comparison by microscopy and denaturing gradient gel electrophoresis. Applied and Environmental Microbiology, 66(2):499-508.
Chen H B, Boutros P C. 2011. VennDiagram:a package for the generation of highly-customizable Venn and Euler diagrams in R. BMC Bioinformatics, 12:35.
Chiriac C M, Szekeres E, Rudi K, Baricz A, Hegedus A, Dragoş N, Coman C. 2017. Differences in temperature and water chemistry shape distinct diversity patterns in thermophilic microbial communities. Applied and Environmental Microbiology, 83(21):e01363-17.
Coman C, Drugă B, Hegedus A, Sicora C, Dragos N. 2013.Archaeal and bacterial diversity in two hot spring microbial mats from a geothermal region in Romania.Extremophiles, 17(3):523-534.
Edgar R C. 2010. Search and clustering orders of magnitude faster than BLAST. Bioinformatics, 26(19):2 460-2 461.
Hahn M W. 2006. The microbial diversity of inland waters.Current Opinion in Biotechnology, 17(3):256-261.
Jia B Y, Tang Y, Tian L Y, Franz L, Alewell C, Huang J H. 2015. Impact of fish farming on phosphorus in reservoir sediments. Scientific Reports, 5:16 617.
Jiang H C, Dong H L, Zhang G X, Yu B S, Chapman L R, Fields M W. 2006. Microbial diversity in water and sediment of Lake Chaka, an athalassohaline lake in northwestern China. Applied and Environmental Microbiology, 72(6):3 832-3 845.
Jiang J G, Shen Y F. 2007. Development of the microbial communities in Lake Donghu in relation to water quality.Environmental Monitoring and Assessment, 127(1-3):227-236.
Joshi A A, Kanekar P P, Kelkar A S, Shouche Y S, Vani A A, Borgave S B, Sarnaik S S. 2008. Cultivable bacterial diversity of alkaline Lonar Lake, India. Microbial Ecology, 55(2):163-172.
Kandel P P, Pasternak Z, Van Rijn J, Nahum O, Jurkevitch E. 2014. Abundance, diversity and seasonal dynamics of predatory bacteria in aquaculture zero discharge systems.FEMS Microbiology Ecology, 89(1):149-161.
Khandeparker L, Kuchi N, Kale D, Anil A C. 2017. Microbial community structure of surface sediments from a tropical estuarine environment using next generation sequencing.Ecological Indicators, 74:172-181.
Kobiyama A, Ikeo K, Reza M S, Rashid J, Yamada Y, Ikeda Y, Ikeda D, Mizusawa N, Sato S, Ogata T, Jimbo M, Kudo T, Kaga S, Watanabe S, Naiki K, Kaga Y, Mineta K, Bajic V, Gojobori T, Watabe S. 2018. Metagenome-based diversity analyses suggest a strong locality signal for bacterial communities associated with oyster aquaculture farms in Ofunato Bay. Gene, 665:149-154.
Korlević M, Šupraha L, Ljubešić Z, Henderiks J, Ciglenečki I, Dautović J, Orlić S. 2016. Bacterial diversity across a highly stratified ecosystem:A salt-wedge Mediterranean estuary. Systematic and Applied Microbiology, 39(6):398-408.
Liu T F, Jia T Z, Chen J N, Liu X Y, Zhao M J, Liu P P. 2017.Analysis of microbial diversity in Shenqu with different fermentation times by PCR-DGGE. Brazilian Journal of Microbiology, 48(2):246-250.
Maloney E M, Liber K, Headley J V, Peru K M, Morrissey C A. 2018. Neonicotinoid insecticide mixtures:Evaluation of laboratory-based toxicity predictions under semicontrolled field conditions. Environmental Pollution, 243:1 727-1 739.
Mwirichia R, Cousin S, Muigai A W, Boga H I, Stackebrandt E. 2011. Bacterial diversity in the haloalkaline Lake Elmenteita, Kenya. Current Microbiology, 62(1):209-221.
Newton R J, Jones S E, Eiler A, McMahon K D, Bertilsson S. 2011. A guide to the natural history of freshwater lake bacteria. Microbiology and Molecular Biology Reviews, 75(1):14-49.
Paul D, Kumbhare S V, Mhatre S S, Chowdhury S P, Shetty S A, Marathe N P, Bhute S, Shouche Y S. 2015. Exploration of microbial diversity and community structure of Lonar Lake:the only hypersaline meteorite crater lake within basalt rock. Frontiers in Microbiology, 6:1 553.
Perkins T L, Clements K, Baas J H, Jago C F, Jones D L, Malham S K, McDonald J E. 2014. Sediment composition influences spatial variation in the abundance of human pathogen indicator bacteria within an estuarine environment. PLoS One, 9(11):e112951.
Pessi I S, Maalouf P D C, Laughinghouse IV H D, Baurain D, Wilmotte A. 2016. On the use of high-throughput sequencing for the study of cyanobacterial diversity in Antarctic aquatic mats. Journal of Phycology, 52(3):356-368.
Roske K, Sachse R, Scheerer C, Röske I. 2012. Microbial diversity and composition of the sediment in the drinking water reservoir Saidenbach (Saxonia, Germany).Systematic and Applied Microbiology, 35(1):35-44.
Smith V H. 2007. Microbial diversity-productivity relationships in aquatic ecosystems. FEMS Microbiology Ecology, 62(2):181-186.
Sogin M L, Morrison H G, Huber J A, Welch D M, Huse S M, Neal P R, Arrieta J M, Herndl G J. 2006. Microbial diversity in the deep sea and the underexplored "rare biosphere". Proceedings of the National Academy of Sciences of the United States of America, 103(32):12 115-12 120.
Valdebenito I I, Gallegos P C, Effer B R. 2015. Gamete quality in fish:evaluation parameters and determining factors.Zygote, 23(2):177-197.
Valdespino-Castillo P M, Alcantara-Hernandez R J, Alcocer J, Merino-Ibarra M, Macek M, Falcón L I. 2014. Alkaline phosphatases in microbialites and bacterioplankton from Alchichica soda lake, Mexico. FEMS Microbiology Ecology, 90(2):504-519.
Venter Z S, Jacobs K, Hawkins H J. 2016. The impact of crop rotation on soil microbial diversity:A meta-analysis.Pedobiologia, 59(4):215-223.
Wang Y, Sheng H F, He Y, Wu J Y, Jiang Y X, Tam N F, Zhou H W. 2012. Comparison of the levels of bacterial diversity in freshwater, intertidal wetland, and marine sediments by using millions of Illumina tags. Applied and Environmental Microbiology, 78(23):8 264-8 271.
Wani A A, Surakasi V P, Siddharth J, Raghavan R G, Patole M S, Ranade D, Shouche Y S. 2006. Molecular analyses of microbial diversity associated with the Lonar soda lake in India:an impact crater in a basalt area. Research in Microbiology, 157(10):928-937.
Wobus A, Bleul C, Maassen S, Scheerer C, Schuppler M, Jacobs E, Röske I. 2003. Microbial diversity and functional characterization of sediments from reservoirs of different trophic state. FEMS Microbiology Ecology, 46(3):331-347.
Wu Q L, Chatzinotas A, Wang J J, Boenigk J. 2009. Genetic diversity of eukaryotic plankton assemblages in Eastern Tibetan Lakes differing by their salinity and altitude.Microbial Ecology, 58(3):569-581.
Zhang L, Hipsey M R, Zhang G X, Busch B, Li H Y. 2017.Simulation of multiple water source ecological replenishment for Chagan Lake based on coupled hydrodynamic and water quality models. Water Science and Technology:Water Supply, 17(6):1 774-1 784.
Zhu L L, Yan B X, Wang L X, Pan X F. 2012. Mercury concentration in the muscle of seven fish species from Chagan Lake, Northeast China. Environmental Monitoring and Assessment, 184(3):1 299-1 310.