Cite this paper:
ZHANG Bo, WU Yingying, WANG Xin, JIANG Wei, YIN Jianping, LIN Qiang. Comparative analysis of mitochondrial genome of a deepsea crab Chaceon granulates reveals positive selection and novel genetic features[J]. Journal of Oceanology and Limnology, 2020, 38(2): 427-437

Comparative analysis of mitochondrial genome of a deepsea crab Chaceon granulates reveals positive selection and novel genetic features

ZHANG Bo1,2,3, WU Yingying1,3,4, WANG Xin1,2, JIANG Wei5, YIN Jianping1,2, LIN Qiang1,2
1 Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510275, China;
2 Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou 510275, China;
3 University of Chinese Academy of Sciences, Beijing 100049, China;
4 Beijing Advanced Sciences and Innovation Center of Chinese Academy of Sciences, Beijing 100049, China;
5 Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Deep-sea organisms survive in an extremely harsh environment. There must be some genetic adaptation mechanisms for them. We systematically characterized and compared the complete mitochondrial genome (mitogenome) of a deep-sea crab (Chaceon granulates) with those of shallow crabs. The mitogenome of the crab was 16 126 bp in length, and encoded 37 genes as most of a metazoan mitogenome, including 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, and 2 ribosomal RNA (rRNA) genes. The gene arrangement and orientation was conserved in the crabs. However, a unique mitogenome element regulator, the origin of light-strand replication (OL), was firstly predicted in the present crab mitogenome. In addition, further positive selection analysis showed that two residues (33S in ND3 and 502I in ND5) in C. granulates mitogenome were positively selected, indicated the selective evolution of the deep-sea crab. Therefore, the mitogenome of deep-sea C. granulates showed a unique OL element and positive selection. These special features would influence the mitochondrial energy metabolism, and be involved in the adaptation of deepsea environment, such as oxygen deficits and low temperatures.
Key words:    deep-sea organisms|mitochondrial genome|adaptation|Chaceon granulates   
Received: 2018-12-18   Revised: 2019-04-04
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