Cite this paper:
LI Zan, LIU Xiumei, DU Xinxin, ZHANG Kai, CHEN Yan, WANG Xubo, WANG Zhigang, YU Haiyang, ZHANG Quanqi. Tumor necrosis factor-alpha (TNF-α) in spotted halibut Verasper variegatus at the embryonic and metamorphic stages[J]. HaiyangYuHuZhao, 2020, 38(2): 454-466

Tumor necrosis factor-alpha (TNF-α) in spotted halibut Verasper variegatus at the embryonic and metamorphic stages

LI Zan1,3, LIU Xiumei2, DU Xinxin3, ZHANG Kai3, CHEN Yan3, WANG Xubo3, WANG Zhigang3, YU Haiyang3, ZHANG Quanqi3
1 School of Agriculture, Ludong University, Yantai 264025, China;
2 College of Life Sciences, Yantai University, Yantai 264005, China;
3 Key Laboratory of Marine Genetics and Breeding, Ministry of Education, College of Marine Life Science, Ocean University of China, Qingdao 266003, China
Abstract:
As an aquatic fish, the spotted halibut Verasper variegatus is highly susceptible to bacterial and virus infections. Tumor necrosis factor-alpha (TNF-α) as a cytokine could control the inflammatory responses. The functions of TNF-α in many species have been widely studied, particularly in mammals. However, little is known about the TNF-α functions in V. variegatus. We first cloned and sequenced the TNF-α gene in V. variegatus (VvTNF-α). The two conserved cysteine residues, transmembrane sequence, Thr-Leu motif, and TNF family signature, as well as the TA-rich motifs of its proteins related to inflammatory responses had high similarity to those of the other teleost and mammalian TNF-α. The phylogenetic analysis showed that VvTNF-α was consistent with TNF-α genes of other vertebrates. The VvTNF-α transcripts were extensively distributed in the peripheral blood leukocytes (PBLs), spleen, and gill, indicating that the VvTNF-α had a role in immune function. Furthermore, treatment with pathogen-associated molecular patterns (PAMPs) could induce a rapid and significant increase of VvTNF-α in the PBLs, which reveals that VvTNF-α does participate in the host immune responses against bacterial and viral pathogens. We found that VvTNF-α had an interesting expression pattern during metamorphosis, showing that the flatfish TNF-α may have some novel functions during specific developmental stages. In addition, the 3D structure prediction of VvTNF-α provided an indication of how it is likely to interact with other proteins. Therefore, VvTNF-α has multiple functions, and provides valuable information to explore novel functions of TNF-α.
Key words:    tumor necrosis factor-alpha (TNF-α)|Verasper variegatus|metamorphosis|peripheral blood leukocytes (PBLs)|3D modeling|pathogen-associated molecular patterns (PAMPs)   
Received: 2018-11-08   Revised: 2019-01-28
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Articles by LI Zan
Articles by LIU Xiumei
Articles by DU Xinxin
Articles by ZHANG Kai
Articles by CHEN Yan
Articles by WANG Xubo
Articles by WANG Zhigang
Articles by YU Haiyang
Articles by ZHANG Quanqi
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