|
|
Cite this paper: |
|
|
ZOU Dandan, TANG Xianghai, QIU Liping, MAO Yunxiang. Defensive physiological characters of Pyropia yezoensis resistant lines to the red rot disease[J]. Journal of Oceanology and Limnology, 2020, 38(2): 509-516 |
|
|
|
|
|
|
|
Defensive physiological characters of Pyropia yezoensis resistant lines to the red rot disease |
|
| ZOU Dandan1,2, TANG Xianghai1,2, QIU Liping1,2, MAO Yunxiang1,2 |
|
1 College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China;
2 Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China |
|
| Abstract: |
| To explore effective physiological indexes to distinguish the resistant lines and susceptible lines of Pythium porphyrae, the causal agent of red rot disease of Pyropia yezoensis, and establish the disease-resistance breeding strategy, we obtained and analyzed the candidate resistant and susceptible lines by population selection. Gametophytes of the candidate lines were cultured in seawater containing Pyt. porphyrae zoospores. Antioxidase activities, including superoxide dismutase (SOD), peroxidase (POD) and polyphenol oxidase (PPO), were measured and compared between the two lines before and after infection. In the resistant lines, SOD and POD activities increased and then decreased, but PPO activity rose with the prolongation of the infection time. The phenylalanine ammonia lyase (PAL) activities also increased and then decreased after infection, but it had significantly different expression in the two lines without pathogen attack. The synthesis rates of β-1,3-glucanase, and cell-wall degrading enzyme were different from each other between the two lines after infection of Pyt. porphyrae. Comparison in the contents of malondialdehyde (MDA) and reactive oxygen species (ROS) in the two lines showed that, the two contents varied synchronously in response to the pathogen attack. Changes of these enzymes activities or contents demonstrated that Pyr. yezoensis could resist against the pathogen of Pyt. porphyrae with the antioxidant defense capacity. In addition, β-1.3-glucanase content showed extremely significant difference between the two lines, and the PAL had consistent expression difference. Therefore, phenylalanine ammonia lyase (PAL) and β-1,3-glucanase can be considered as an effective index to distinguish susceptible line and resistant line, with which the workload of the resistant breeding could be reduced in the future. |
|
| Key words:
population selection|resistant lines|susceptible lines|Pyropia yezoensis|Pythium porphyrae
|
|
| Received: 2019-05-23 Revised: 2019-07-11 |
|
|
|
|
References:
Abeles F B, Bosshart R P, Forrence L E. 1971. Preparation and purification of glucanase and chitinase from bean leaves.Plant Physiology, 47(1):129-134.
Albersheim P, Valent B S. 1974. Host-pathogen interactions VII. Plant pathogens secrete proteins which inhibit enzymes of the host capable of attacking the pathogen. Plant Physiology, 53(5):684-687.
Aoki Y, Kamei Y. 2006. Preparation of recombinant polysaccharide-degrading enzymes from the marine bacterium, Pseudomonas sp. ND137 for the production of protoplasts of Porphyra yezoensis. European Journal of Phycology, 41(3):321-328.
Apostol I, Heinstein P F, Low P S. 1989. Rapid stimulation of an oxidative burst during elicitation of cultured plant cells role in defense and signal transduction. Plant physiology, 90(1):109-116.
Baucher M, Bernard-Vailhé M A, Chabbert B et al. 1999.Down-regulation of cinnamyl alcohol dehydrogenase in transgenic alfalfa (Medicago sativa L.) and the effect on lignin composition and digestibility. Plant Molecular Biology, 39(3):437-447.
Bouarab K, Adas F, Gaquerel E et al. 2004. The innate immunity of a marine red alga involves oxylipins from both the eicosanoid and octadecanoid pathways. Plant Physiology, 135(3):1 838-1 848.
Chen, G X, Asada K. 1989. Ascorbate peroxidase in tea leaves:occurrence of two isozymes and the differences in their enzymatic and molecular properties. Plant and Cell Physiology, 30(7):987-998.
Collén J, Hervé C, Guisle-Marsollier I et al. 2006. Expression profiling of Chondrus crispus (Rhodophyta) after exposure to methyl jasmonate. Journal of Experimental Botany, 57(14):3 869-3 881.
Foyer C H, Descourvieres P, Kunert K J. 1994. Protection against oxygen radicals:an important defense mechanism studied in transgenic plants. Plant Cell and Environment, 17(5):507-523.
Friend J, Reynolds S B, Aveyard M A. 1973. Phenylalanine ammonia lyase, chlorogenic acid and lignin in potato tuber tissue inoculated with Phytophthora infestans. Physiological Plant Pathology, 3(4):495-507.
Kawamura Y, Yokoo K, Tojo M et al. 2005. Distribution of Pythium porphyrae, the causal agent of red rot disease of Porphyrae spp., in the Ariake Sea, Japan. Plant Disease, 89(10):1 041-1 047.
Koca H, Bor M, Özdemir F et al. 2007. The effect of salt stress on lipid peroxidation, antioxidative enzymes and proline content of sesame cultivars. Environmental and Experimental Botany, 60(3):344-351.
Koukol J, Conn E E. 1961. The metabolism of aromatic compounds in higher plants IV. Purification and properties of the phenylalanine deaminase ofHordeum vulgare. Journal of Biological Chemistry, 236(10):2 692-2 698.
Küpper F C, Kloareg B, Guern J et al. 2001. Oligoguluronates elicit an oxidative burst in the brown algal kelp Laminaria digitata. Plant Physiology, 125(1):278-291.
Li F L, Fu Y, Yuan Q, Lü W H, Xu Y Q, Liu R M, Hu B Z, Chen F W, Xu Y Y, Feng Y Z. 2015. Variations in antioxidase activities and MDA content in potato tubers infected by Fusarium trichothecioides. Agricultural Science and Technology, 16(11):2 433-2 436.
Liang Y, Chen Q, Liu Q et al. 2003. Exogenous silicon (Si)increases antioxidant enzyme activity and reduces lipid peroxidation in roots of salt-stressed barley (Hordeum vulgare L.). Journal of Plant Physiology, 160(10):1 157-1 164.
Mauch F, Hadwiger L A, Boller T. 1984. Ethylene:symptom, not signal for the induction of chitinase and β-1,3-glucanase in pea pods by pathogens and elicitors. Plant Physiology, 76(3):607-611.
Mittler R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science, 7(9):405-410.
Park C S, Hwang E K. 2014. Isolation and evaluation of a strain of Pyropia yezoensis (Bangiales, Rhodophyta)resistant to red rot disease. Journal of Applied Phycology, 26(2):811-817.
Park C S, Kakinuma M, Amano H. 2001. Detection and quantitative analysis of zoospores of Pythium porphyrae, causative organism of red rot disease in Porphyra, by competitive PCR. Journal of Applied Phycology, 13:433-441.
Peng M, Kuc J. 1992. Peroxidase-generated hydrogen peroxide as a source of antifungal activity in vitro and on tobacco leaf disks. Phytopathology, 82(6):696-699.
Shao H B, Jiang S Y, Li F M et al. 2007. Some advances in plant stress physiology and their implications in the systems biology era. Colloids and Surfaces B:Biointerfaces, 54(1):33-36.
Stefani F O, Tanguay P, Pelletier G et al. 2010. Impact of endochitinase-transformed white spruce on soil fungal biomass and ectendomycorrhizal symbiosis. Applied and Environmental Microbiology, 76(8):2 607-2 614.
Veitía N, García L R, Bermúdez-Caraballoso I et al. 2014.Field resistance of selected potato clones to early blight. Biotecnología Vegetal, 14(4):215-221.
Weinberger F, Leonardi P, Miravalles A et al. 2005. Dissection of two distinct defense-related responses to agar oligosaccharides in Gracilaria chilensis (Rhodophyta)and Gracilaria conferta (Rhodophyta). Journal of Phycology, 41(4):863-873.
Werner S, Steiner U, Becher R et al. 2002. Chitin synthesis during in planta growth and asexual propagation of the cellulosic oomycete and obligate biotrophic grapevine pathogen Plasmopara viticola. FEMS Microbiology Letters, 208(2):169-173.
Woo J H, Kitamura E, Myouga H et al. 2002. An antifungal protein from the marine bacterium Streptomyces sp. strain AP77 is specific for Pythium porphyrae, a causative agent of red rot disease in Porphyra spp. Applied and Environmental Microbiology, 68(6):2 666-2 675.
Wu C T, Bradford K J. 2003. Class I chitinase and β-1,3-glucanase are differentially regulated by wounding, methyl jasmonate, ethylene, and gibberellin in tomato seeds and leaves. Plant Physiology, 133(1):263-273.
Zhang X N, Sun P P, Mao Y X et al. 2015. The variation of defensive compounds content and protective enzymes activity of Pyropia yezoensis gametophyte infected by oomycete Pythium porphyrae. Journal of Ocean University of China (Natural Science), 45(12):57-64. (in Chinese with English abstract)
|
|
|
|