Cite this paper:
LI Yongfu, LI Ruiqian, YI Xiaoyan. Effects of light quality on growth rates and pigments of Chaetoceros gracilis (Bacillariophyceae)[J]. HaiyangYuHuZhao, 2020, 38(3): 795-801

Effects of light quality on growth rates and pigments of Chaetoceros gracilis (Bacillariophyceae)

LI Yongfu1,2,3, LI Ruiqian4, YI Xiaoyan5
1 Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;
2 Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China;
3 Nantong Research and Development Center of Marine Science and Technology, Institute of Oceanology, Chinese Academy of Sciences, Nantong 226019, China;
4 School of International Affairs and Public Administration, Ocean University of China, Qingdao 266100, China;
5 Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, China
Light is an important parameter in algal culturing. In this work, the effects of different light qualities on growth of the marine diatom Chaetoceros gracilis are evaluated. The cells were cultured under light quantum flux density of 60 μmol photons/(m2·s) with nine light qualities: LED red light (LR), LED blue light (LB), LED red plus LED blue light (LR+LB), LED white light (LW), fluorescent white light (FW), and combinations of LW and FW lights with increased proportions of red or blue light (FW+LR, FW+LB, LW+LR, and LW+LB). Blue light promoted the growth of C. gracilis largely. Three light qualities, FW+LR, LW+LR, and LR, resulted in the lowest growth rate. Both chlorophyll and carotenoids reached the highest level under LB and the lowest level under LR among monochromatic light sources; however, increasing of the proportion of blue or red light in the white light induced an increase of the chlorophyll and carotenoid concentrations. Light absorption ability of microalgae was critical for the growth of these organisms, as we observed a positive correlation between the extinction coefficient at different wavelengths and the specific growth rate. These findings are of importance to improve the culturing of this alga in bioreactors.
Key words:    Chaetoceros gracilis|light quality|pigments|wavelength effect|mean extinction coefficients   
Received: 2019-06-28   Revised: 2019-08-19
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