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CHEN Na, LI Li, LI Chenghua, LIN Zhihua, MENG Jie, LIU Sheng, SONG Kai, BAO Yongbo. bHLH genes polymorphisms and their association with growth traits in the Pacific oyster Crassostrea gigas[J]. HaiyangYuHuZhao, 2020, 38(3): 862-868

bHLH genes polymorphisms and their association with growth traits in the Pacific oyster Crassostrea gigas

CHEN Na1,2, LI Li3, LI Chenghua2, LIN Zhihua1, MENG Jie3, LIU Sheng3, SONG Kai3, BAO Yongbo1
1 Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315121, China;
2 School of Marine Sciences, Ningbo University, Ningbo 315211, China;
3 Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
The basic helix-loop-helix (bHLH) genes encode a large superfamily of transcription factors in the Pacific oyster (Crassostrea gigas), and play a very important role in regulation of growth and development. To investigate the oyster growth traits and the associations with bHLH genes variations, we analyzed the gene polymorphisms-traits association in a wild population, and confirmed the results in another independent wild population by targeted gene re-sequencing and SNPshot analysis. After screening the single nucleotide polymorphisms (SNPs) in three candidate genes of the bHLH family (88 bHLH genes in two wild oyster populations in total), we identified the allele CgLoblHLH4-T/G located in the exon of the CgLoblHLH4 gene. This allele is a non-synonymous mutation (Phe/Leu) with an extremely significant association with shell width (P<0.01) and allele G is beneficial to shell width. This SNP on the CgLoblHLH4 gene might have a potential value as a genetic marker of growth traits that could be used in breeding in C. gigas in the future.
Key words:    Crassostrea gigas|basic helix-loop-helix (bHLH)|re-sequencing|single nucleotide polymorphism (SNP) shot|growth traits   
Received: 2019-03-16   Revised: 2019-07-02
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