Cite this paper:
JIANG Fenghua, ZHANG Li, YANG Baijuan, ZHENG Li, SUN Chengjun. Biomarker responses in the bivalve Chlamys farreri to the water-soluble fraction of crude oil[J]. Journal of Oceanology and Limnology, 2015, 33(4): 853-861

Biomarker responses in the bivalve Chlamys farreri to the water-soluble fraction of crude oil

JIANG Fenghua1,2, ZHANG Li1,2, YANG Baijuan1,2, ZHENG Li1,2, SUN Chengjun1,2
1 Marine Ecology Center, First Institute of Oceanography, State Oceanic Administration (SOA), Qingdao 266061, China;
2 Key Lab of Active Material and Modern Analysis Technology, State Oceanic Administration (SOA), Qingdao 266061, China
To investigate the effect of the water soluble fraction of crude oil (WSF) on marine bivalves, the scallop Chlamys farreri was exposed to three WSF concentrations (0.18 mg/L, 0.32 mg/L, and 0.51 mg/L, respectively) in seawater. Petroleum hydrocarbon contents in scallops and a suite of enzymes [7-Ethoxyresorufin-O-deethylase (EROD), aryl hydrocarbon hydroxylase (AHH), glutathione S-transferase (GST), and glutathione peroxidase (GPx)] in gills and digestive glands were monitored over 10 days. The results revealed that WSF affected the activity of the four enzymes in the gills and digestive glands. EROD activity in the gills was significantly induced in most individuals of the three test groups, while in the digestive gland it was significantly induced in the low-concentration group within 4 days but was inhibited in the middle- and high-concentration groups on days 1, 4, and 10. AHH activity in the gills of all treatment groups was significantly induced on day 1. In the digestive gland, AHH activity was induced in most individuals from the treatment groups. In all treatment groups, GST activity was significantly inhibited from days 2 to 10 in the gills and was induced after day 4 in the digestive gland. GPx activity in the gills was significantly inhibited throughout the exposure period in all treatment groups. There was no overall significant difference in GPx activity in the digestive gland between the control and treatment groups. Our results also revealed that petroleum hydrocarbon concentrations in the tissues increased linearly with exposure time. EROD activity in the digestive gland and GST and GPx activity in the gill tissue were negatively correlated with petroleum hydrocarbon body burden. These enzymes play important roles in detoxification and can act as potential biomarkers for monitoring petroleum hydrocarbon contaminants in the marine environment.
Key words:    crude oil water soluble fraction (WSF)|Chlamys farreri|biomarkers|detoxification   
Received: 2014-06-18   Revised: 2014-11-13
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