Isolation and characterization of halophilic bacteria and archaea from salt ponds in Hangu Saltworks, Tianjin, China -- Journal of Oceanology and Limnology,2015,33(4):862-868

	Cite this paper:
	DENG Yuangao, XU Gaochao, SUI Liying. Isolation and characterization of halophilic bacteria and archaea from salt ponds in Hangu Saltworks, Tianjin, China[J]. Journal of Oceanology and Limnology, 2015, 33(4): 862-868

Isolation and characterization of halophilic bacteria and archaea from salt ponds in Hangu Saltworks, Tianjin, China

DENG Yuangao, XU Gaochao, SUI Liying

Tianjin Key Laboratory of Marine Resources & Chemistry, Tianjin University of Science & Technology, TEDA, Tianjin 300457, China

Abstract:

A total of 26 isolates were obtained from solar salt ponds of different salinities (100, 150, 200, and 250) in Hangu Saltworks Co. Ltd., Tianjin, China. Phylogenetic analysis of 16S rRNA gene sequences indicated that five bacteria genera Halomonas, Salinicoccus, Oceanobacillus, Gracibacillus, and Salimicrobium and one archaea genera Halorubrum were present. The genus Halomonas was predominant with eight strains distributed in a salinity range of 100-200, followed by Halomonas with six strains in salinity 250. Based on the genus and original sampling salinity, eight bacterial and two archaeal isolates were selected for further morphological, physiological, and biochemical characterization. All of the bacterial strains were moderately halophilic with the optimal salinity for growth being either 50 or 100, while two archaeal strains were extremely halophilic with an optimal growth salinity of 200. Additionally, we put forth strain SM.200-5 as a new candidate Salimicrobium species based on the phylogenic analysis of the 16S rRNA gene sequence and its biochemical characteristics when compared with known related species.

Key words: halophilic bacteria|halophilic archaea|isolation|salinity|salt ponds

Received: 2014-07-25 Revised: 2014-11-12

Tools

PDF ( KB) Free

Print this page

Add to favorites

Email this article to others

Authors

Articles by DENG Yuangao

Articles by XU Gaochao

Articles by SUI Liying

References:
Antón J, Rosselló-Mora R, Rodríguez-Valera F, Amann R. 2000. Extremely halophilic bacteria in crystallizer ponds from solar salterns. Appl. Environ. Microbiol., 66 (7): 3 052-3 057.
Asker D, Ohta Y. 1999. Production of canthaxanthin by extremely halophilic bacteria. J. Biosci. Bioengin., 88 (6): 617-621.
Asker D, Ohta Y. 2002. Haloferax alexandrinus sp. nov., an extremely halophilic canthaxanthin-producing archaeon from a solar saltern in Alexandria (Egypt). J. Syst. Evol. Microbiol., 52 (3): 729-738.
Chen L, Wang G Y, Bu T, Zhang Y B, Liu M, Zhang J, Lin X K. 2010. Identification of a moderately halophilic bacterium whb45 and screening of its antimicrobial and antitumor activity. Microbio l ogy China, 37 (1): 85-90. (in Chinese with English abstract)
Chenna R, Sugawara H, Koike T, Lopez R, Gibson T J, Higgins D G, Thompson J D. 2003. Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Res., 31 (13): 3 497-3 500.
Cui H L, Lin Z Y, Dong Y, Zhou P J, Liu S J. 2007. Halorubrum litoreum sp. nov., an extremely halophilic archaeon from a solar saltern. J. Syst. Evol. Microbiol., 57 (10): 2 204- 2 206.
Fang C J, Ku K L, Lee M H, Su N W. 2010. Influence of nutritive factors on C 50 carotenoids production by Haloferax mediterranei ATCC 33500 with two-stage cultivation. Biores. Technol., 101 (16): 6 487-6 493.
Javor B J. 2002. Industrial microbiology of solar salt production. J. Ind. Microbial. Biotechnol., 28 (1): 42-47.
Jones A G, Ewing C M, Melvin M V. 1981. Biotechnology of solar saltfields. Hydrobiologia, 81-82 : 391-406.
Litchfield C D. 2011. Potential for industrial products from the halophilic Archaea. J. Ind. Microbiol. Biotechnol., 38 (10): 1 635-1 647.
Lizama C, Monteoliva-Sánchez M, Prado B, Ramos- Cormenzana A, Weckesser J, Campos V. 2001. Taxonomic study of extreme halophilic archaea isolated from the “Salar de Atacama”, Chile. J. Syst. Appl. Microbiol., 24 (3): 464-474.
Montalvo-Rodríguez R, Ruíz-Acevedo A, López-Garriga J. 1997. New isolates of extremely halophilic Archaebacteria (Halobacteria) from Puerto Rico and the Caribbean. Caribbean J. Sci., 33 (1-2): 98-104.
Olsen G J. 1994. Microbial ecology. Archaea, archaea, everywhere. Nature, 731 : 657-658.
Oren A. 2002. Diversity of halophilic microorganisms: environments, phylogeny, physiology, and applications. J. Ind. Microbiol. Biotechnol., 28 (1): 237-243.
Oren A, Rodríguez-Valera F. 2001. The contribution of halophilic bacteria to the red coloration of saltern crystallizer ponds. FEMS Microbiol. Ecol., 36 (2-3): 123- 130.
Quillaguamán J, Hashim S, Bento F, Mattiasson B, Hatti-Kaul R. 2005. Poly (β-hydroxybutyrate) production by a moderate halophile, Halomonas boliviensis LC1 using starch hydrolysate as substrate. J. Appl. Microbiol., 99 (1): 151-157.
Pašić L, Bartual S G, Ulrih N P, Grabnar M, Velikonja B H. 2005. Diversity of halophilic archaea in the crystallizers of an Adriatic solar saltern. FEMS Microbiol. Ecol., 54 (3): 491-498.
Pathak A P, Sardar A G. 2012. Isolation and characterization of carotenoid producing Haloarchaea from solar saltern of Mulund, Mumbai, India. Indian J. Nat. Prod. and Res., 3 (4): 483-488.
Rodríguez-Valera F, Ventosa A, Juez G, Imhoff J F. 1985. Variation of environmental features and microbial populations with salt concentrations in a multi-pond saltern. Microbial Ecol., 11 (2): 107-115.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol., 28 (10): 2 731-2 739.
Tan D, Xue Y S, Aibaidula G, Chen G Q. 2011. Unsterile and continuous production of polyhydroxybutyrate by Halomonas TD01. Biores. Technol., 102 (17): 8 130- 8 136.
Vreeland R H, Litchfield C D, Martin E L, Elliot E. 1980. Halomonas elongata, a new genus and species of extremely salt-tolerant bacteria. Int. J. Syst. Bacteriol., 30 (2): 485-495.
Wais A C. 1988. Recovery of halophilic archaebacteria from natural environments. FEMS Microbiol. Ecol., 53 (3-4): 211-216.
Yeon S H, Jeong W J, Park J S. 2005. The diversity of culturable organotrophic bacteria from local solar salterns. J. Microbiol., 43 (1): 1-10.
Yoon J H, Kang S J, Oh K H, Oh T K. 2009. Salimicrobium flavidum sp. nov., isolated from a marine solar saltern. J. Syst. Evol. Microbiol., 59 (Pt 11): 2 839-2 842.
Zahran H H. 1997. Diversity, adaptation and activity of the bacterial flora in saline environments. Biol. Fertil. Soils, 25 (3): 211-223.