Cite this paper:
QIU Lixia, YU Zhiming, CAO Xihua, JI Hena, SONG Xiuxian. The mechanism of a new type of modified clay controlling Phaeocystis globosa growth[J]. Journal of Oceanology and Limnology, 2020, 38(4): 1270-1282

The mechanism of a new type of modified clay controlling Phaeocystis globosa growth

QIU Lixia1,2,3,4, YU Zhiming1,2,3,4, CAO Xihua1,2,4, JI Hena1,2,3,4, SONG Xiuxian1,2,3,4
1 CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;
3 University of Chinese Academy of Sciences, Beijing 100049, China;
4 Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
Phaeocystis globosa is a harmful algal bloom (HAB) species worldwide. Using modified clay (MC) to control HABs and to mitigate their adverse effects is currently a commonly used method in China. In this paper, the effects of oxidized composite modified clay (OXI-MC) on P. globosa were studied from different perspectives. The results show that the OXI-MC could effectively remove P. globosa and inhibit both the growth of residual algal cells and the formation of new colonies. The P. globosa algal biomass removal efficiencies after 3 h reached 90% at a dose of 0.1 g/L, and the number of colonies with different particle sizes was greatly reduced. Compared with those of the control, the superoxide dismutase (SOD) activity, catalase (CAT) activity, and malondialdehyde (MDA) content of the residual algae significantly increased, indicating that OXI-MC caused oxidative stress in the algal cells. In addition, we evaluated the effects of OXI-MC on the photosynthesis of residual microalgae and found that the maximal photochemical efficiency of photosystem Ⅱ (PSⅡ) under dark adaptation (Fv/Fm) and actual photochemical efficiency of PSⅡ (ФPSⅡ) decreased, severely damaging the photosynthesis efficiency, implying that OXI-MC effected the photosynthesis system of P. globosa. The results of this study clarify that OXI-MC could remove the most of algal cells and break up the colonies of P. globosa by collision, flocculation, and releasing active substances, as well as inhibit effectively the growth and colony formation of residual P. globosa by causing oxidative stress, reducing photosynthesis activity, accelerating the degradation of polysaccharides, and inhibiting the formation of colonies.
Key words:    Phaeocystis globosa|modified clay|colony disruption|oxidative stress|photosynthesis   
Received: 2020-01-19   Revised: 2020-03-19
PDF (2307 KB) Free
Print this page
Add to favorites
Email this article to others
Articles by QIU Lixia
Articles by YU Zhiming
Articles by CAO Xihua
Articles by JI Hena
Articles by SONG Xiuxian
Alvarez M E, Pennell R I, Meijer P, Ishikawa A, Dixon R A, Lamb C. 1998. Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity. Cell, 92(6): 773-784.
Anipsitakis G P, Tufano T P, Dionysiou D D. 2008. Chemical and microbial decontamination of pool water using activated potassium peroxymonosulfate. Water Research, 42(12): 2 899-2 910.
Asada K. 2006. Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiology, 141(2): 391-396.
Cao X H, Yu Z M, Qiu L X. 2017. Field experiment and emergent application of modified clays for Phaeocystis globosa blooms mitigation. Oceanologia et Limnologia Sinica, 48(4): 753-759. (in Chinese with English abstract)
Chen S G, Strasser R J, Qiang S. 2014. In vivo assessment of effect of phytotoxin tenuazonic acid on PSⅡ reaction centers. Plant Physiology and Biochemistry, 84: 10-21.
Demple B, Halbrook J, Linn S. 1983. Escherichia coli xth mutants are hypersensitive to hydrogen peroxide. Journal of Bacteriology, 153(2): 1 079-1 082.
Domingues N, Matos A R, Marques da Silva J, Cartaxana P. 2012. Response of the diatom Phaeodactylum tricornutum to photooxidative stress resulting from high light exposure. PLoS One, 7(6): e38162,
Foyer C H, Noctor G. 2005. Redox homeostasis and antioxidant signaling: a metabolic interface between stress perception and physiological responses. The Plant Cell, 17(7): 1 866-1 875.
Granum E, Myklestad S M. 1999. Effects of NH4+ assimilation on dark carbon fixation and β-1,3-glucan metabolism in the marine diatom Skeletonema costatum (Bacillariophyceae).Journal of Phycology, 35(6): 1 191-1 199.
Guan C W, Guo X Y, Li Y, Zhang H J, Lei X Q, Cai G J, Guo J J, Yu Z M, Zheng T L. 2015. Photoinhibition of Phaeocystis globosa resulting from oxidative stress induced by a marine algicidal bacterium Bacillus sp. LP-10. Scientific Reports, 5(1): 17002.
Guillard R R L, Hargraves P E. 1993. Stichochrysis immobilis is a diatom, not a chrysophyte. Phycologia, 32(3): 234-236.
Gururani M A, Venkatesh J, Tran L S P. 2015. Regulation of photosynthesis during abiotic stress-induced photoinhibition. Molecular Plant, 8(9): 1 304-1 320.
Huang T W, Wang X D, Wang Y. 2012. Growth, architecture and cell distribution in Phaeocystis globosa colonies.Chinese Bulletin of Botany, 47(5): 508-514. (in Chinese with English abstract)
Jiang Z J, Huang X P, Zhang J P. 2013. Dynamics of nonstructural carbohydrates in seagrass Thalassia hemprichii and its response to shading. Acta Oceanologica Sinica, 32(8): 61-67.
Liu J S, Yang W D, Zhang H, Wu Y. 2006. Effects of chlorine dioxide on contents of chlorophyll a, proteins and DNA in Phaeocystis globosa. Journal of Tropical and Subtropical Botany, 14(5): 427-432. (in Chinese with English abstract)
Liu S Y, Yu Z M, Song X X, Cao X H. 2017. Effects of modified clay on the physiological and photosynthetic activities of Amphidinium carterae Hulburt. Harmful Algae, 70: 64-72.
Liu S Y, Yu Z M, Song X X, Cao X H. 2018. Physiological and photosynthetic responses of Karenia mikimotoi to the modified clay mitigation method. Marine Pollution Bulletin, 133: 491-499.
Liu Y, Cao X H, Yu Z M, Song X X, Qiu L X. 2016. Controlling harmful algae blooms using aluminum-modified clay. Marine Pollution Bulletin, 103(1-2): 211-219.
Lu G Y, Song X X, Yu Z M, Cao X H, Yuan Y Q. 2015. Effects of modified clay flocculation on major nutrients and diatom aggregation during Skeletonema costatum blooms in the laboratory. Chinese Journal of Oceanology and Limnology, 33(4): 1 007-1 019.
Lu G Y, Song X X, Yu Z M, Cao X H. 2017. Application of PAC-modified kaolin to mitigate Prorocentrum donghaiense: effects on cell removal and phosphorus cycling in a laboratory setting. Journal of Applied Phycology, 29(2): 917-928.
Ma B H, Gao L, Zhang H X, Cui J, Shen Z G. 2012. Aluminuminduced oxidative stress and changes in antioxidant defenses in the roots of rice varieties differing in Al tolerance. Plant Cell Reports, 31(4): 687-696.
Mittler R, Vanderauwera S, Gollery M, Van Breusegem F. 2004. Reactive oxygen gene network of plants. Trends in Plant Science, 9(10): 490-498.
Nagata T. 2000. Production mechanisms of dissolved organic matter. In: Kirchmann DL ed. Microbial Ecology of the Oceans. Wiley-Liss, New York, p.121-152.
Paetzold S C, Davidson J. 2011. Aquaculture fouling: efficacy of potassium monopersulphonate triple salt based disinfectant (Virkon® Aquatic) against Ciona intestinalis.Biofouling, 27(6): 655-665.
Parsons T. 1984. A Manual of chemical & biological methods for seawater analysis. Pergamon Press, 31: 158-161.
Qi Y Z, Xu N, Wang Y, Lv S H, Chen J F. 2002. Progress of studies on red tide in China-studies on Phaeocystis globosa red tide and its DMS (DMSP) production. China Basic Science, (4): 23-28. (in Chinese with English abstract)
Qin X L, Lai J X, Chen B, Jiang F J, Xu M B. 2016. Molecular identification of Phaeocystis from Beibu Gulf based on 18S rDNA sequences. Journal of Tropical and Subtropical Botany, 24(2): 176-181. (in Chinese with English abstract)
Qiu L X, Yu Z M, Cao X H, Song X X, Liu Y, Zhong Y. 2017.Removal efficiencies for Phaeocystis globosa and Prorocentrum donghaiense with modified clay.Oceanologia et Limnologia Sinica, 48(5): 982-989. (in Chinese with English abstract)
Sharma V K. 2002. Potassium ferrate (VI): an environmentally friendly oxidant. Advances in Environmental Research, 6(2): 143-156.
Shen P P, Wang Y, Qi Y Z, Xie L C, Lv S H, Hodgkiss I J. 2000.Growth characteristics and life cycle of Phaeocystis globosa Scherffel. Acta Hydrobiologica Sinca, 24(6):635-643. (in Chinese with English abstract)
Smith D C, Steward G F, Long R A, Azam F. 1995. Bacterial mediation of carbon fluxes during a diatom bloom in a mesocosm. Deep Sea Research Part Ⅱ: Topical Studies in Oceanography, 42(1): 75-97.
Stirbet A, Govindjee. 2011. On the relation between the Kautsky effect (chlorophyll a fluorescence induction) and photosystem Ⅱ: basics and applications of the OJIP fluorescence transient. Journal of Photochemistry and Photobiology B: Biology, 104(1-2): 236-257.
Su X W, D’Souza D H. 2012. Inactivation of human norovirus surrogates by benzalkonium chloride, potassium peroxymonosulfate, tannic acid, and Gallic acid.Foodborne Pathogens and Disease, 9(9): 829-834.
Van Boekel W H M. 1992. Phaeocystis colony mucus components and the importance of calcium ions for colony stability. Marine Ecology Progress Series, 87:301-305.
van Rijssel M, Janse I, Noordkamp D J B, Gieskes W W C. 2000. An inventory of factors that affect polysaccharide production by Phaeocystis globosa. Journal of Sea Research, 43(3-4): 297-306.
Waites W M, Wyatt L R, King N R, Bayliss C E. 1976. Changes in spores of Clostridium bifermentans caused by treatment with hydrogen peroxide and cations. Journal of General Microbiology, 93(2): 388-396.
Wang Z F, Yu Z M, Song X X, Cao X H, Liu K. 2014b. Impact of modified clay on the growth of the infant Apostichopus japonicas Selenka in HABs controling. Oceanologia et Limnologia Sinica, 45(2): 233-238. (in Chinese with English abstract)
Wang Z F, Yu Z M, Song X X, Cao X H. 2014a. Effects of modified clay on the infant of Patinopecten yessoensis for HABs control. Marine Environmental Science, 33(6):817-821, 836. (in Chinese with English abstract)
Yu Z M, Sengco M R, Anderson D M. 2004. Flocculation and removal of the brown tide organism, Aureococcus anophagefferens (Chrysophyceae), using clays. Journal of Applied Phycology, 16(2): 101-110.
Yu Z M, Song X X, Cao X H, Liu Y. 2017. Mitigation of harmful algal blooms using modified clays: theory, mechanisms, and applications. Harmful Algae, 69: 48-64.
Yu Z M, Zou J Z, Ma X N. 1994. Application of clays to removal of red tide organisms Ⅱ. Coagulation of different species of red tide organisms with montmorillonite and effect of clay pretreatment. Chinese Journal of Oceanology and Limnology, 12(4): 316-324.
Zhang S F, Zhang K, Cheng H M, Lin L, Wang D Z. 2020.Comparative transcriptomics reveals colony formation mechanism of a harmful algal bloom species Phaeocystis globosa. Science of the Total Environment, 719: 137454,
Zhang Y, Song X X, Yu Z M, Zhang P P, Cao X H, Yuan Y Q. 2019. Impact assessment of modified clay on embryolarval stages of turbot Scophthalmus maximus L. Journal of Oceanology and Limnology, 37(3): 1 051-1 061.
Zhang Y, Yu Z M, Song X X, Yuan Y Q, Cao X H. 2018. Effects of modified clay used for the control of harmful algal blooms on Alexandrium pacificum cysts. Harmful Algae, 72: 36-45.
Zhao T, Tan L J, Huang W Q, Wang J T. 2019. The interactions between micro polyvinyl chloride (mPVC) and marine dinoflagellate Karenia mikimotoi: the inhibition of growth, chlorophyll and photosynthetic efficiency.Environmental Pollution, 247: 883-889.
Zhu J N, Yu Z M, He L Y, Cao X H, Ji H N, Song X X. 2019.Physiological response dynamics of the brown tide organism Aureococcus anophagefferens treated with modified clay. Harmful Algae, 86: 1-9.
Zhu J N, Yu Z M, He L Y, Cao X H, Liu S Y, Song X X. 2018.Molecular mechanism of modified clay controlling the brown tide organism Aureococcus anophagefferens revealed by transcriptome analysis. Environmental Science & Technology, 52(12): 7 006-7 014.
Copyright © Haiyang Xuebao