Cite this paper:
LIN Chenggang, LIU Xiaolu, SUN Lina, LIU Shilin, SUN Jingchun, ZHANG Libin, YANG Hongsheng. Effects of different flow velocities on behavior and TRPA1 expression in the sea cucumber Apostichopus japonicas[J]. Journal of Oceanology and Limnology, 2020, 38(4): 1328-1340

Effects of different flow velocities on behavior and TRPA1 expression in the sea cucumber Apostichopus japonicas

LIN Chenggang1,2,3,4, LIU Xiaolu1,2,3,5, SUN Lina1,2,3, LIU Shilin1,2,3, SUN Jingchun1,2,3, ZHANG Libin1,2,3, YANG Hongsheng1,2,3,4
1 CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2 Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;
3 Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;
4 The Innovation of Seed Design, Chinese Academy of Sciences, Wuhan 430071, China;
5 College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
A water flow simulation device capable of adjusting flow velocity was designed in flow velocity range of 0-30 cm/s, with which an indoor experiment was conducted to simulate the movement and adhesive behaviors of different-sized Apostichopus japonicus under different flow velocities. Observation showed that, in slow flow (~5 cm/s), A. japonicus moved more distance than in still water, and hardly moved in the riptide (~30 cm/s); and the adhesive capacity of A. japonicus was related to the flow velocity and attachment time. A. japonicus were able to attach to the bottom after any attachment time in the slow flow, after 10 s in the medium flow (~15 cm/s), and after 60 s in the riptide (~30 cm/s). In addition, larger A. japonicus were stronger with adhesive ability than smaller ones. The transcriptome data showed that the expression of transient receptor potential cation channel subfamily A, member 1 (TRPA1) in the tube feet was increased significantly in a flowing water, but those in the tentacles and tube feet were not significantly changed. Fluorescence in-situ hybridization results showed that TRPA1 was expressed around the watervascular of tentacles, tube feet, body wall, and spines. Therefore, tube feet were important for sea cucumbers to keep themselves stable in relatively swift flow with adhesion ability.
Key words:    sea cucumber|Apostichopus japonicus|behavior|flow velocity|transient receptor potential cation channel subfamily A, member 1 (TRPA1)   
Received: 2020-02-25   Revised: 2020-04-04
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