Cite this paper:
LI Yanyu, ZHANG Tao, ZHANG Congyao, ZHU Ying, DING Jianfeng, MA Yuexin. Bacterial diversity in the intestine of young farmed puffer fish Takifugu rubripes[J]. Journal of Oceanology and Limnology, 2015, 33(4): 913-918

Bacterial diversity in the intestine of young farmed puffer fish Takifugu rubripes

LI Yanyu1, ZHANG Tao2, ZHANG Congyao1, ZHU Ying1, DING Jianfeng1, MA Yuexin1
1 Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China;
2 Dalian Tianzheng Industry Co. Ltd., Dalian 116011, China
The aim of the study was to examine the bacterial community associated with the intestinal mucus of young farmed puffer fish Takifugu rubripes. Polymerase chain reaction and partial 16S rDNA sequencing was performed on DNA from bacteria cultivated on Zobell 2216E medium. All the isolates were classified into two phyla—Proteobacteria and Firmicutes. Proteobacteria were the dominant, culturable intestinal microbiota (68.3%). At the genus level, Vibrio, Enterobacter, Bacillus, Pseudomonas, Exiguobacterium, Staphylococcus, Acinetobacter, Pseudoalteromonas and Shewanella were isolated from the intestine, with representatives of the genera Vibrio, Enterobacter and Bacillus accounting for 70.7% of the total. This is the first report of Enterobacter, Bacillus, Exiguobacterium and Staphylococcus as part of the intestinal bacterial microflora in T. rubripes. The profile of the culturable bacterial community differed between samples collected from the same tank at 2-month intervals, as indicated by Bray-Curtis and Sorensen indices, and the impact on the intestinal physiology and health of puffer fish requires further investigation.
Key words:    16S rDNA|biodiversity|intestinal microflora|Takifugu rubripes   
Received: 2014-09-03   Revised: 2015-01-20
PDF ( KB) Free
Print this page
Add to favorites
Email this article to others
Articles by LI Yanyu
Articles by ZHANG Tao
Articles by ZHANG Congyao
Articles by ZHU Ying
Articles by DING Jianfeng
Articles by MA Yuexin
Bray J R, Curtis J T. 1957. An ordination of the upland forest communities of southern Wisconsin. Ecol. Monogr., 27 (4): 325-349.
Burbank D R, Shah D H, LaPatra S E, Fornshell G, Cain K D. 2011. Enhanced resistance to coldwater disease following feeding of probiotic bacterial strains to rainbow trout (Oncorhynchus mykiss). Aquaculture, 321 (3-4): 185-190.
Chen C H, Tanaka Y, Komiya Y, Itoi S, Sugita H. 2013. Predominant intestinal bacteria of the pufferfish (Takifugu rubripes Temminck & Schlegel, 1850) reared in an indoor tank as determined by the clone library analysis and culture method. J. Appl. Ichthyol., 29 (6): 1 374-1 377.
Gao L J, Huang Y Q, Xia L J, Lu J X, Liu S C. 2011. Comparison of flesh quality of farmed fugu, Takifugu rubripes from different culture models. J. F ish. C hina, 35 (11): 1 668- 1 676. (in Chinese with English abstract)
Gómez G D, Balcázar J L. 2008. A review on the interactions between gut microbiota and innate immunity of fish. FEMS Immunol. Med. Microbiol., 52 (2): 145-154.
Kim D H, Brunt J, Austin B. 2007. Microbial diversity of intestinal contents and mucus in rainbow trout (Oncorhynchus mykiss). J. Appl. Microbiol., 102 (6): 1 654-1 664.
Lane D J. 1991. 16S/23S rRNA sequencing. In : Stackebrandt E, Goodfellow M D eds. Nucleic Acid Techniques in Bacterial Systematic. 1stedn. John Wiley and Sons, Chichester. p.115-175.
Liu Z M, Ma Y X, Yang Z P, Li M, Liu J, Bao P Y. 2012. Immune responses and disease resistance of the juvenile sea cucumber Apostichopus japonicus induced by Metschnikowia sp. C14. Aquaculture, 368 -369 : 10-18.
Luczkovich J J, Stellwag E J. 1993. Isolation of cellulolytic microbes from the intestinal tract of the pinfish, Lagodon rhomboides : size-related changes in diet and microbial abundance. Mar. Biol., 116 (3): 381-388.
Merrifield D L, Dimitroglou A, Foey A, Davies S J, Baker R T M, Bøgwald J, Castex M, Ringø E. 2010. The current status and future focus of probiotic and prebiotic applications for salmonids. Aquaculture, 302 (1-2): 1-18.
Noguchi T, Hwang D F, Arakawa O, Sugita H, Deguchi Y, Shida Y, Hashimoto K. 1987. Vibrio alginolyti c us, a tetrodotoxin-producing bacterium, in the intestines of the fish Fugu vermicularis vermi c ularis. Mar. Biol., 94 (4): 625-630.
Pielou E C. 1966. The measurement of diversity in different types of biological collections. J. T heor. B iol., 13 : 131-144.
Shannon C E. 1948. A mathematical theory of communication. The Bell Syst em Techn ical J ournal, 27 (3): 379-423.
Shiina A, Itoi S, Washio S, Sugita H. 2006. Molecular identification of intestinal microflora in Takifugu niphobles. Comp. Biochem. Physiol. Part D, 1 (1): 128- 132.
Sørensen T. 1948. A method of establishing groups of equal amplitude in plant sociology based on similarity of species content, and its application to analyses of the vegetation on Danish commons. Kongelige Danske Videnskabernes Selskabs Biologiske Skrifter, 5 (4): 1-34.
Sugita H, Iwata J, Miyajima C, Kubo T, Noguchi T, Hashimoto K, Deguchi Y. 1989. Changes in microflora of a puffer fish Fugu niphobles, with different water temperatures. Mar. Biol., 101 (3): 299-304.
Sugita H, Noguchi T, Furuta M, Harada T, Murata O, Hashimoto K, Deguchi Y. 1988. The intestinal microflora of cultured specimens of a puffer Fugu rubripes rubripes. Nippon Suis an Gakkaishi, 54 (4): 733.
Sugita H, Noguchi T, Hwang D F, Furuta M, Motokane T, Sonoda T, Hashimoto K, Deguchi Y. 1987. Intestinal microflora of coastal puffer fishes. Nippon Suisan Gakkaishi, 53 (12): 2 201-2 207.
Sugita H, Sugiyama K, Itoi S. 2010. Culturable bacterial flora in the intestinal tract of Japanese pufferfish Takifugu rubripes. Aquacult. Sci., 58 (3): 437-438.
Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol., 24 (8): 1 596-1 599.
Tanasomwang V, Muroga K. 1989. Intestinal microflora of rockfish Sebastes schlegeli, tiger puffer Takifugu rubripes and red grouper Epinephelus akaara at their larval and juvenile stages. Nippon Suisan Gakkaishi, 55 (8): 1 371- 1 377.
Tapia-Paniagua S T, Díaz-Rosales P, León-Rubio J M, de La Banda I G, Lobo C, Alarcón F J, Chabrillón M, Rosas-Ledesma P, Varela J L, Ruiz-Jarabo I, Arijo S, Esteban M A, Martínez-Manzanares E, Mancera J M, Balebona M C, Moriñigo M A. 2012. Use of the probiotic Shewanella putrefaciens Pdp11 on the culture of Senegalese sole (Solea senegalensis, Kaup 1858) and gilthead seabream (Sparus aurata L.). Aquacult. Int., 20 (6): 1 025-1 039.
Wu S G, Gao T H, Zheng Y Z, Wang W W, Cheng Y Y, Wang G T. 2010. Microbial diversity of intestinal contents and mucus in yellow catfish (Pelteobagrus fulvidraco). Aquaculture, 303 (1-4): 1-7.
Wu Z L, Yang Y, Xie L P, Xia G L, Hu J C, Wang S J, Zhang R Q. 2005. Toxicity and distribution of tetrodotoxinproducing bacteria in puffer fish Fugu rubripes collected from the Bohai Sea of China. Toxicon, 46 (4): 471-476.
Yang G M, Bao B L, Peatman E, Li H R, Huang L B, Ren D M. 2007. Analysis of the composition of the bacterial community in puffer fish Takifugu obscurus. Aquaculture, 262 (2-4): 183-191.
Copyright © Haiyang Xuebao