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LI Xinxin, FENG Xiuni, LUO Kai, MA Shuoli, DENG Junming, ZHANG Wenbing, MAI Kangsen. Effects of vitamin C deficiency or excess on growth performance, anti-oxidative response and fatty acid composition of juvenile abalone Haliotis discus hannai Ino[J]. Journal of Oceanology and Limnology, 2020, 38(6): 1936-1944

Effects of vitamin C deficiency or excess on growth performance, anti-oxidative response and fatty acid composition of juvenile abalone Haliotis discus hannai Ino

LI Xinxin1, FENG Xiuni2, LUO Kai1, MA Shuoli1, DENG Junming3, ZHANG Wenbing1, MAI Kangsen1
1 Key Laboratory of Mariculture(Ministry of Education), Key Laboratory of Aquaculture Nutrition and Feeds(Ministry of Agriculture), Fisheries College, Ocean University of China, Qingdao 266003, China;
2 Jiaozhou Fisheries Technology Promotion Station, Qingdao 266003, China;
3 College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China
A 240-day feeding trial was conducted to investigate the effects of dietary vitamin C on growth performance, anti-oxidative response, and fatty acid composition of juvenile abalone Haliotis discus hannai Ino (initial body weight:0.93±0.00 g). Three semi-purified experimental diets were formulated containing 0.00, 94.52, and 9 649.58 mg/kg of vitamin C supplied as L-ascorbyl-2-monophosphate. The results show that there was no significant difference in weight gain ratio, daily increment in shell length, and survival rate among the three treatments. Adding dietary vitamin C (9 649.58 mg/kg) significantly increased the activities of superoxide dismutase (SOD), glutathine peroxidase (GPX), glitathione-S-transferase (GST), glutathione reductase (GR), alkaline phosphatase (AKP), and lysozyme in viscera (P<0.05). In muscle, activities of phenoloxidase, catalase, SOD, GST, GR, and AKP were increased in the treatment with 9 649.58 mg/kg of dietary vitamin C (P<0.05). The highest concentrations of ascorbic acid in viscera and muscle were found in the group with 9 649.58 mg/kg of dietary vitamin C (P<0.05). The contents of crude protein and crude lipid in the soft body were significantly increased in the 9 649.58 mg/kg group (P<0.05). Dietary vitamin C supplementation significantly decreased the contents of saturated fatty acids (14:0, 16:0, and 18:0), and increased the composition of 18:2n-6 and 22:6n-3 in the soft body of abalone (P<0.05). Therefore, although there were no significant effects on the growth performance, dietary vitamin C supplementation improved the anti-oxidation and immune responses, increased specific unsaturated fatty acid (i.e., 16:1, 18:1n-7, 18:1n-9, 18:2n-6 and 22:6n-3), and decreased specific saturated fatty acid (i.e., 14:0, 16:0 and 18:0) contents in the soft body of abalone.
Key words:    abalone|ascorbic acid|anti-oxidative capability|fatty acid   
Received: 2019-08-03   Revised: 2019-09-05
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Articles by LI Xinxin
Articles by FENG Xiuni
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Articles by MAI Kangsen
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