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ZHANG Xinxu, WU Huijuan, LI Zhongzhen, LI Yuanyou, WANG Shuqi, ZHU Dashi, WEN Xiaobo, LI Shengkang. Effects of dietary supplementation of Ulva pertusa and nonstarch polysaccharide enzymes on gut microbiota of Siganus canaliculatus[J]. HaiyangYuHuZhao, 2018, 36(2): 438-449

Effects of dietary supplementation of Ulva pertusa and nonstarch polysaccharide enzymes on gut microbiota of Siganus canaliculatus

ZHANG Xinxu1,2, WU Huijuan1,2, LI Zhongzhen1,2, LI Yuanyou1,2, WANG Shuqi1,2, ZHU Dashi1,2, WEN Xiaobo1,2, LI Shengkang1,2
1 Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China;
2 Marine Biology Institute, Shantou University, Shantou 515063, China
Fishes represent the highest diversity of vertebrates; however, our understanding of the compositions and functions of their gut microbiota is limited. In this study, we provided the first insight into the gut microbiota of the herbivorous fish Siganus canaliculatus by using three molecular ecology techniques based on the 16S rRNA genes (denaturing gradient gel electrophoresis, clone library construction, and highthroughput Illumina sequencing), and the Illumina sequencing technique is suggested here due to its higher overall coverage of the total 16S rRNA genes. A core gut microbiota of 29 bacterial groups, covering >99.9% of the total bacterial community, was found to be dominated by Proteobacteria and Firmicutes in fish fed three different diets with/without the supplementation of Ulva pertusa and non-starch polysaccharide (NSP) enzymes (cellulase, xylanase, and β-glucanase). Diverse potential NSP-degrading bacteria and probiotics (e.g., Ruminococcus, Clostridium and Lachnospiraceae) were detected in the intestine of the fish fed U. pertusa, suggesting that these microorganisms likely participated in the degradation of NSPs derived from U. pertusa. This study supports our previous conclusion that U. pertusa-based diets are suitable for the production of S. canaliculatus with lower costs without compromising quality.
Key words:    aquaculture|gut microbiota|Siganus canaliculatus|16S rRNA gene|Ulva pertusa   
Received: 2016-09-19   Revised:
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